Serum separation for Dengue IgM antibodies in the blood Samples

. PURPOSE: This SOP explains the procedure for separation of serum from blood samples

2. STAFF RESPONSIBLE AND AUTHORIZED TO PERFORM THE PROCEDURE: Laboratory technicians

3. PRINCIPLE OF PROCEDURE USED FOR EXAMINATION – Not applicable

4. PROCEDURE

1.     After receiving, serum is separated by centrifugation at 1500 rpm for 10 minutes.

2.     Each test tube containing blood sample is put in the centrifuge and is balanced by keeping tube of another blood sample or test tube of plain water (dummy tube) if there is not another sample.

3.     Centrifuge is covered with lid.

4.     Centrifuge is put on at 1500 rpm for 10 minutes.(Refer SOP No 2.3, Page no 13,14 of Insrtruments, SOP issue no 3)

5.     After 10 minutes, centrifuge is put off & allowed to come to complete halt on its own.

6.     Stopping the centrifuge in between with hands leads to disturbance of RBCs & clear serum is not obtained.

7.     Turbid, lipaemic, and hemolysed samples are discarded and a repeat sample is requested.

8.     After the serum is separated, the test tubes of blood sample are put back in rack for ELISA testing.

5. PERFORMANCE SPECIFICATION – Not applicable

6. SAMPLE – Blood sample

7. TYPE OF CONTAINER – plain test tube, plain Vacutte

8. MATERILS, EQUIPMENTS AND REAGENTS USED

• Table top Centrifuge,
• Blood samples in plain test tube,
• Test tube rack
• Gloves

9. REVIEW, RECORDS, REMARKS AND RECOMMENDATION:

·       Register no DR 08– Log Book of Centrifuge

10.  REFERENCE –

·       Manual of Laboratory Techniques, NICD, chapter no 4.2.1.4 .2 Page no-25