Distribution of authentic sabin virus reference standard from NIBSC

1.     Scope

• This procedure explains how to distribute authentic sabin virus reference strain received    from ERC, Mumbai. From this prepared vials, lab standard of virus are made to check cell sensitivity.

2.     Definition

·       ml        =          Milliliter.

·       ART    =          Aerosol Resistant Tips.

3.    Material

• 5 ampoules of each sabin virus P1, P2, and P3 received from ERC –Bombay on 30/1/2003

·       Pippeters with ART Tips

·       Sterile 1.8ml externally threaded vials

·       Storage box for keeping distributed vials

4.    Procedure

·       All received ampoules were frozen at -20⁰C.

·       Take two ampoules (0.8 ml in each ampoule) of Authentic Sabin Virus Reference Standard Type 1.

·       Thaw and mix using ART tips and distribute 0.1ml in each labeled sterile 1.8ml externally threaded vials.

·       Close the cap tightly and store at -20⁰C. Approximately 16 aliquots are formed.

·       Perform the same steps for Standard Type 2 and Type 3.

5.    Safety conditions

·       Work only one serotype at a time in biosafety cabinet.

·       To avoid any leakage tighten the tubes properly.

6.    Documentation

·       NIBSC & Lab standard Sabin virus register-PR 40

7.    References used to draw SOP

·       WHO Polio Laboratory Manual.