Functioning and Maintenance of Centrifuge

2.3   CENTRIFUGE

Details:

Name of the Equipment	Centrifuge No-2
Name of the Company	Remi
Model No.	R303-4-92
Serial no	DMLC-1018
Year of Purchase	1999
Year of First use	1999
Inventory card no.	BJMC/Micro/Viro/CHA/Centrifuge/1999/2
Approximate Price	Rs. 5000/-
Maintenance by	Bio Medical Work shop
Location	Dengue lab
Responsibility	Laboratory Technicians
Record Maintained in	DR 08, DF 16

PURPOSE: - This is used for centrifuging blood samples to separate serum or plasma.

LOCATION – Dengue Lab

DEFINITION – RPM- Revolutions per minute.

OPERATION

1. It should be placed horizontally on even surface to avoid accidental displacement.
2. Centrifuge tubes are balanced always with dummy tubes filled equally with water kept on the opposite side.
3. Switch on and increase or decrease speed gradually.
4. Always cover it with lid before starting it.
5. Stop immediately if it makes abnormal noise.
6. After the centrifugation is over, put off the switch and allow the centrifuge to stop spontaneously. Do not try to stop manually.

MAINTENANCE:

·       After use invert buckets to drain dry.

·       After any spillage, fill the buckets or any area with 1% Hypochlorite, keep for 30 minutes then remove it, wipe with sterile cotton.

·       Clean the centrifuge daily as it is most frequently used instrument.

·       Check for corrosion and repaint if necessary.

RESPONSIBILITY – Laboratory Technicians

SAFETY PRECAUTIONS:

• Do not use without balance on opposite side
• Do not stop it suddenly.
• Do not use without plugging the test tube mouth.
• Speed should not be raised rapidly.
• Stop immediately if there is breakage of tube. (Refer Safety Manual, Issue no.1, Section-13, Page no.62)

REVIEW, RECORDS, REMARKS & RECOMMENDATIONS:

1. DR 08  Log book of centrifuge machine
2. DF 16  Calibration file

           

REFERENCE:

1. National Institute of Communicable Diseases, Manual of laboratory Techniques; Chapter-6 ‘Maintenance of Equipments’,  Page: 209.
2. Cheesbrough M, District Laboratory practice in Tropical Countries, Part 1, 2005; Chapter 4.7 ‘Centrifuge’; Page: 138 - 143